Generation of Human IPSC-Derived Neuron-Astrocyte-Microglia Tri-Cultures and Their Application in Pharmacology and Toxicity Assays
- CNS drug evaluation requires a shift from neuron‑only models to systems that incorporate glial contributions
- Introduce the generation of neurotoxic aged astrocytes derived from human iPSCs and present the results of drug screening conducted using these cells
- Demonstrate the potential application of the neurotoxic aged astrocyte model for novel therapeutic target identification through a combination of proprietary gene expression network analysis and siRNA screening
- Show the potential utility of a neuron-astrocyte-microglia tri-culture system for pharmacology and toxicity assays